Research Article

A Seamless Gene Deletion Method and Its Application for Regulation of Higher Alcohols and Ester in Baijiu Saccharomyces cerevisiae

Table 1

Strains and plasmids used in the current study.

Strains or plasmidsRelevant characteristicReference or source

Strains
DH5αsupE44 ∆lacU169 (φ 80lacZ∆M15) hsdR17 recAl endAl gyrA96 thi1 relAThis lab
yWH245ade2::HERP1.0 ∆leu2 ura3 Δho::KanMXGifted by William G
α5MATα, haploid yeast strain from AY15This lab
Hα5 (50)MATα, Δbat2::S-HERP1.0-BAT2-U2This study
Hα5 (150)MATα, Δbat2::S-HERP1.0-BAT2-U3This study
Hα5 (300)MATα, Δbat2::S-HERP1.0-BAT2-U4This study
Hα5 (500)MATα, Δbat2::S-HERP1.0-BAT2-UThis study
Hα5 (700)MATα, Δbat2::S-HERP1.0-BAT2-U5This study
Hα5 (1000)MATα, Δbat2::S-HERP1.0- BAT2-U6This study
Hα5+HMATα, Δbat2::S-HERP1.0-BAT2-UThis study
Hα5MATα, Δbat2This study
Hα5::ATF1+HMATα, Δbat2::S-HERP1.0-BA--ATF1-This study
Hα5::ATF1MATα, Δbat2:: -ATF1-This study
Plasmids
pUC19, cloning vectorThis lab
Yep352, ori control vectorThis lab
pUC-BBTAP, containing BA-PTEF1-ATF1-TPGK1-BB cassetteThis lab
pUC19-UD, containing BAT2-UD cassetteThis study
YHERP1.0, , containing HERP1.0 cassetteThis study
YHERP1.0ΔBAT2, , containing HERP1.0-BAT2-UD cassetteThis study
YHERP1.0(50 bp), , containing HERP1.0-BAT2-U2D cassetteThis study
YHERP1.0(150 bp), , containing HERP1.0-BAT2-U3D cassetteThis study
YHERP1.0(300 bp), , containing HERP1.0-BAT2-U4D cassetteThis study
YHERP1.0(500 bp), , containing HERP1.0-BAT2-UD cassetteThis study
YHERP1.0(700 bp), , containing HERP1.0-BAT2-U5D cassetteThis study
YHERP1.0(1000 bp), , containing HERP1.0-BAT2-U6D cassetteThis study
YHERP1.0ΔBAT2::ATF1, , containing HERP1.0-BA --ATF1--BB cassetteThis study