Research Article
A Seamless Gene Deletion Method and Its Application for Regulation of Higher Alcohols and Ester in Baijiu Saccharomyces cerevisiae
Table 2
Primers used in the present study.
| | Primers | Sequence () | Restriction site |
| | BAT2-A(KpnI)-F | GGGGTACCCTCTACCACCTGCTGCAG | KpnI | | BAT2-A-R | TACGTAGCAATAGCGATACTATCGTTCTTAAAACTCGTGG | | | BAT2-B-F | CCACGAGTTTTAAGAACGATAGTATCGCTATTGCTACGTA | | | BAT2-B(SphI)-R | ACATGCATGCTTATTTTCCGTCAATTTTCAATCTTGCGCCC | SphI | | YH1.0(EcoRI)-F | GACCATGATTACGAATTCTGGATGGACGCAAAGAAG | EcoRI | | Yh1.0(SphI)-R | GTGCCAAGCTTGCATGCGGGGTACCATTAAGGGTTCTCGAGAGCT | SphI | | KpnI | | B+50 bp-F | GGGGTACCAAAATTTTAGAAATTTAAGG | KpnI | | B+150 bp-F | GGGGTACCGCTGACAGTATAACTAATAT | KpnI | | B+300 bp-F | GGGGTACCCCCTCTCTGACACCTCTTGT | KpnI | | B+500 bp-F | GGGGTACCCGCTCCTTTCCAAACATCTT | KpnI | | B+700 bp-F | GGGGTACCACAAGAACATCTCATCTACT | KpnI | | B+1000 bp-F | GGGGTACCCCACCAGTTTCACGCCTACC | KpnI | | BAT2-B-R | GGGGTACCCCTCAATCGGCACATTCATA | KpnI | | BAT2-A-F | GCTCCCTCCAACTACTCT | | | BAT2-A-R(F) | AACTTCTTTGCGTCCATCCAAGTCGACATTACCCTGTTATCCCTAATCGTTCTTAAAACTCGTGG | I-SCEI | | BAT2-B-F(R) | CCACGAGTTTTAAGAACGATTAGGGATAACAGGGTAATGTCGACTTGGATGGACGCAAAGAAGTT | I-SCEI | | AHB-A-U | TTCACTGGGACCCTTTCA | | | AHB-A-D | GCTTCTAATCCGTACTTCAATA | | | AHB-B-U | ATGCGTCAATCGTATGTG | | | AHB-B-D | TGAAATCCTTGTCCAGCT | | | YH1.0ΔB::A-F | CGAGAACCCTTAATGGTACCGCTCCTTTCCAAACATCTTCG | KpnI | | YH1.0ΔB::A-R | AAGCTTGCATGCGGGGTACCGCCCTCTAAAGATTCATCGG | KpnI | | AHB(ATF1)-A-D | GCATCGGGCTCCTCTAACTG | | | AHB(ATF1)-B-U | GGGTTCAATATACAAGGCTTCG | |
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