A Seamless Gene Deletion Method and Its Application for Regulation of Higher Alcohols and Ester in Baijiu Saccharomyces cerevisiae

Table 3

The alcohol and residual sugar content fermented by the parental strain and engineered strains .

Strains	Alcohol content (%, v/v)	Residual sugar (g/L)	Total acids (g/L)	Total esters (g/L)
α5	16.03 ± 0.15	2.04 ± 0.12	0.093 ± 0.012	0.705 ± 0.009
Hα5	16.12 ± 0.11	2.88 ± 0.10	0.100 ± 0.015	0.629 ± 0.011
Hα5::ATF1	15.67  ± 0.10	3.01 ± 0.08	0.075  ± 0.010	1.490  ± 0.015

Data are the average of three independent experiments ± the standard deviation. Significant difference of Hα5::ATF1 from the parental strain was confirmed by Student’s t-test (P < 0.05, n = 3).