Research Article

SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae)

Table 4

Combinations of species-specific primers (Calliphoridae).

Combination NamePrimer SetSequence ()Optimized Concentration
(μM)
Target Species

CC1mgCTT TGA TCC AGC AGG AGG AG
TGA AGT GAA ATA AGC TCG
TGT GTC T
0.25Ch. megacephala
il/caGGA TTC GTT CAT TGA TAT CCT
C
ATC GTC GTG GTA TTC CTG CT
0.30L. illustris
L. caesar
reTTG GAG GAT TTG GAA ATT GA
GGG AAA GCT ATA TCA GGT
GCC
0.35P. regina

CC2laGTA GTT GCT CAT TTT CAT TAT
GTG C
GGG TAT CAG TGT ACA AAT
CCT GCT
0.20C. lata
amTTC TAC AAT TGG ATC AAC
AAT TTC TC
AAC AAG ACT TTC TCA CAC
AAT AAA GAA
0.20L. ampullacea
caGGA AAG AAG GAA ACA TTT
GGG
TTG AGT TCC GTA AAG AGT
TGC T
0.15L. caesar

CC3grTGA CTT CTA CCC CCT GCA C
CAG TTC ATC CTG TTC CAG CTC
0.15A. graham
(L. ampullacea)
viCTG TCC CAA CAG GAA TTA
AGA TTT TT
TTG GGT ACC ATA AAG AGT
TGC T
0.30C. vicina
voATA CGA TCA ACA GGT ATT
ACC TTC
CTC CTG CTG GGT CAA AGA AT
1.50C. vomitoria

CC4piCGT AGA TAC TCG AGC TTA
TTT CAC C
TCT TAA TTC CAG TTG GTA
CAG CA
0.10Ch. pinguis
seGAA ACA TTC GGT TCA TTA
GGG ATG
GCT CGT GTA TCA ACG TCT
ATT CC
1.50L. sericata
clATA TGG CTT TCC CTC GGA
TAA AC
GCT CCT CCA TGT GCG ATA TT
0.10T. calliphoroides
(C. vicina)