SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae)

<table class="fixed-width table-group" id="tab7"><tr><td><table class="table"><colgroup><col style="width:9.25em"/><col style="width:8.66em"/><col style="width:7.96em"/></colgroup><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Combination Name</td><td class="align_center">Amplified Species</td><td class="align_center"><i>T</i><sub><i>m</i></sub> of Amplicon <br/>(°C)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left" rowspan="2">CS1</td><td class="align_center"><i>S. peregrina</i></td><td class="align_center">75.15±0.46</td></tr><tr><td class="align_center"><i>S. melanura</i></td><td class="align_center">79.99±0.22</td></tr><tr><td class="align_left" colspan="3"><hr/></td></tr><tr><td class="align_left" rowspan="2">CS2</td><td class="align_center"><i>S. dux</i></td><td class="align_center">74.20±0.95</td></tr><tr><td class="align_center"><i>S. similis</i></td><td class="align_center">77.77±0.52</td></tr><tr><td class="align_left" colspan="3"><hr/></td></tr><tr><td class="align_left" rowspan="3">CS3</td><td class="align_center"><i>S. haemorrhoidalis</i></td><td class="align_center">74.05±0.21</td></tr><tr><td class="align_center"><i>S. crassipalpis</i></td><td class="align_center">75.75±0.31</td></tr><tr><td class="align_center"><i>S. albiceps</i></td><td class="align_center">79.19±0.59</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Obtained melting temperatures (<i>T</i><sub><i>m</i></sub>) of amplicons using primer combinations (Sarcophagidae).</div>

BioMed Research International

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Table 7

Table 7: SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae) 

Table 7 | SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae) 