Minocycline Protects against Rotenone-Induced Neurotoxicity Correlating with Upregulation of Nurr1 in a Parkinson’s Disease Rat Model

Figure 3

Expression of tyrosine hydroxylase (TH) and Nurr1 in the SN of rats. (a) Double immunostaining for TH and Nurr1 in the SN of rats (×400; green, TH; red, Nurr1; blue, staining with DAPI; scale bar = 50 um). (b) The number of immunostaining positive cells for TH and Nurr1 in the SN of rats (n=5 for each group). (c) The protein expression of TH and Nurr1 in the SN of rats determined by western blot analysis. (d) The relative band intensities of TH and Nurr1 normalized to the expression of β-actin (n=5 for each group). <0.05, compared to the control group; ^#p<0.05, compared to the rotenone-alone-treated group.

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