FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens

Figure 5

E₂ or FSH treatment of chicken granulosa cells. (a) FOXO3 mRNA levels after treatment of chicken granulosa cells with different concentrations of E₂. (b) ERβ mRNA levels after treatment with E₂ at different doses. (c) Western blot analysis showing ERβ and FOXO3 expression levels after treatment with E₂ (β-actin served as a reference). (d) FOXO3 mRNA levels after treatment of chicken granulosa cells with different FSH concentrations. (e) FSHR mRNA levels after treatment with FSH at different doses. (f) Western blot analysis showing the amounts of FSHR and FOXO3 proteins after treatment with FSH (β-actin served as a reference). Data are expressed as means ± SEM (n = 3 independent cell cultures). P<0.05; P<0.01; P<0.001.

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