Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA

Table 4

Extraction efficiency and total yield of DNA when three different types of binding buffers were tested at three different polymer weights.

Binding buffer	Polymer weight	Concentration (ng/μL)	260/280 wavelength ratio	DNA yield (ng)	Extraction capacity ng/μL	Extraction efficiency (%)
2 M GuHCl/ EtOH	0.02 g	633	1.431	316500	1582.5	11.4%
2 M NaCl	0.02 g	126	1.589	63000	315	2.2%
PBS	0.02 g	51.4	0.613	25700	128.5	0.93%
2 M GuHCl/ EtOH	0.10 g	832	1.499	416000	2080	15.0%
2 M NaCl	0.10 g	397	1.520	198500	992.5	7.1%
PBS	0.10 g	322	1.322	161000	805	5.8%
2 M GuHCl/EtOH	0.20 g	899	1.601	449500	2247.5	16.2%
2 M NaCl	0.20 g	677	1.682	338500	1692.5	12.2%
PBS	0.20 g	350	1.491	175000	875	6.3%