Biochemical Hallmarks of Oxidative Stress-Induced Overactivation of Xenopus Eggs

Figure 3

Oxidative stress-induced accumulation of lipofuscin in Xenopus eggs. Spot assay of lipofuscin autofluorescence in the insoluble particulate fractions of the peroxide-treated eggs and its quantification are presented in panels (a) and (b), respectively. The items labeled as 480 refer to control eggs incubated for 480 min in the absence of peroxide. Stars in panel (b) indicate statistical significance from the untreated control (p<0.05). Panel (c) shows SBB staining of the egg endosomal compartment. Hydrogen peroxide (f.c. 100 mM) was added to the eggs at 0 time.

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(b)

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