IGHG1 Regulates Prostate Cancer Growth via the MEK/ERK/c-Myc Pathway

Figure 2

Inhibition of IGHG1 suppresses the growth of PCa cells. (a) Cultured DU145 cells were transfected with IGHG1 siRNA fragment, and the siRNA efficiency was confirmed by western blot. Then the DU145 (b) and PC3 (c) cells were subjected to MTS assay to evaluate the cell growth. The data of 0 h, 12 h, 24 h, and 48 h after transfection was shown as the growth rate. And the cells were stained with PI and Annexin-V to reveal cell cycle (d) and apoptosis (g) by flow cytometry technique. The statistical data were shown in (e), (h) in DU145 cells and (f), (i) in PC3 cells. All experiments were performed in triplicate, and results are expressed as the mean ± SD. denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.

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