High Improvement in Lactic Acid Productivity by New Alkaliphilic Bacterium Using Repeated Batch Fermentation Integrated with Increased Substrate Concentration

Table 3

Effect of different glucose concentrations on growth and kinetic parameters of lactic acid production by Enterococcus hirae BoM1-2.

Conc. of glucose (g L^−1)		LA conc. (g L^−1)	(g g^−1)	(g L^−1 h^−1)	Residual sugar (g L^−1)	Fermentation time (h)	Max (g L^−1 h^−1)
20	1.99±0.009	19.6±0.1	0.98	2.18	0.1±0.1	9	3.2 (3h)
40	4.19±0.021	37.3±0.5	0.93	2.07	0.0±0.0	18	3.3 (3h)
60	6.25±0.120	57.9±0.1	0.97	1.75	0.0±0.0	33	3.4 (3h)
80	7.69±0.074	77.4±0.0	0.97	1.23	0.5±0.1	63	3.1 (3h)
100	9.19±0.160	96.0±0.1	0.96	1.07	0.0±0.0	90	3.3 (3h)
150	9.72±0.064	109.9±0.4	0.96	1.02	35.7±0.4	108	2.6 (3h)

OD, maximum optical density. Maximum lactic acid concentration. Lactic acid yield. Lactic acid productivity at the end of fermentation time. Maximum lactic acid productivity at indicated time. Different letters between columns denote that mean values are significantly different (p≤0.05) by Tukey test; values are means ± SE (n=3).