Dihydrochalcone Derivative Induces Breast Cancer Cell Apoptosis via Intrinsic, Extrinsic, and ER Stress Pathways but Abolishes EGFR/MAPK Pathway

Figure 4

Reduction of mitochondrial transmembrane potential (MTP) of MDA-MB-231 and MCF-7 cells after treatment with compound 1. MDA-MB-231 and MCF-7 cells were treated with compound 1 at various concentrations for 24 hours, and MTP was measured by staining with DiOC₆ and employing flow cytometry. The relative fluorescence intensity was measured. Histograms (a) and bar graphs (b) of percentage of both cancer cells with losses of MTP are presented. The statistical significance values compared to the control (without treatment) are marked with asterisks, , , , and .

(a)

(b)