Dihydrochalcone Derivative Induces Breast Cancer Cell Apoptosis via Intrinsic, Extrinsic, and ER Stress Pathways but Abolishes EGFR/MAPK Pathway

Figure 7

Alteration of apoptotic Bcl-2 family proteins and cell cycle checkpoint mRNA expressions in compound 1-treated MDA-MB-231 and MCF-7 cells. MDA-MB-231 and MCF-7 cells were treated with compound 1 at various concentrations for 12 hours. The expression levels of BH3-only proteins Bim, Bad, and Bid together with the truncated form (tBid) were demonstrated by Western blotting (a). The bands were folds when compared with the control as verified by using β-actin as a constitutive protein. The bar graphs of the relative levels of protein expressions of treated MDA-MB-231 cells (b) and MCF-7 cells (c) were obtained using densitometry from three independent experiments with the same results. Bar graphs are representative of atm and atr gene expressions in compound 1-treated MDA-MB-231 cells (d) and MCF-7 cells (e). The statistical significance values compared to the control (without treatment) are marked with asterisks, and .

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