[Retracted] MicroRNA-205-5p Targets HMGB1 to Suppress Inflammatory Responses during Lung Injury after Hip Fracture

Figure 1

Determination of successful hip fracture in a Sprague-Dawley (SD) rat and of HMGB1 as the target of miR-205-5p. (a) Representative X-ray image of a SD rat model of hip fracture and control (1 : 1). (b) Representative hematoxylin and eosin-stained lung tissue sections and representative tissue sections stained immunohistochemically to detect HMGB1 are shown in the top and bottom panels, respectively (magnification, 40×). (c) Representative fluorescent images of tissues subjected to the TUNEL assay. Green and blue areas represent apoptotic cells and cell nuclei, respectively (magnification, 40×). (d) The relative expression levels of miR-205-5p and HMGB1 and IL-6 mRNA in the hip fracture and control groups were determined using qPCR. , , and vs. the control. (e) Western blot analysis of the levels of HMGB1, p65, p-IkBα/IkBα, and RAGE. Equal protein loading was verified using GAPDH or Lamin A as an internal control. (f) Relative levels of miR-205-5p and HMGB1 in serum samples from human patients relative to controls. ; . (g) Correlation analysis between the levels of miR-205-5p and HMGB1 in human serum samples. (h) Predicted conserved miR-205-5p binding sites in HMGB1 mRNA. (i) Luciferase activity analysis of cells transfected with constructs encoding wild-type or mutated HMGB1 in the presence of miR-205-5p or NC. WT-3′UTR (+) indicates wild-type HMGB 3′UTR. WT-3′UTR (-) indicates mutant-3′UTR HMGB1. Mimics indicates miR-205-5p mimics. vs. the control.

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