[Retracted] MicroRNA-205-5p Targets HMGB1 to Suppress Inflammatory Responses during Lung Injury after Hip Fracture

Figure 2

Effects of a transfected miR-205-5p mimic or inhibitor in HPAEpiC cells. (a) The relative expression levels of miR-205-5p and HMGB1 were determined by qPCR. , , and vs. the other two groups. (b) CCK8 assay of cell proliferation. The results represent the means of three independent experiments. and vs. the other two groups. (c) Western blot analysis of the levels of HMGB1, p65, p-IkBα/IkBα, and RAGE. Equal protein loading was verified using GAPDH or Lamin A as internal controls. (d) The levels of HMGB1 and p65 were determined via immunofluorescence analysis (magnification, LSM 120×). (e) Representative micrographs of EdU-labeled samples from three groups (magnification, 10×) and (f) corresponding fluorescence images of samples stained with Hoechst 33258 (magnification, 20×). (g) Flow cytometric detection of apoptotic cells in samples double-stained with Annexin V-FITC and PI. vs. the other groups. (h) Images of a cell cycle analysis as determined by PI staining and flow cytometry. vs. the other groups. (i) The concentrations of HMGB1, IL-6, and TNF-α in cell supernatants were determined by ELISA. , , and vs. the other groups.

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