[Retracted] MicroRNA-205-5p Targets HMGB1 to Suppress Inflammatory Responses during Lung Injury after Hip Fracture

Figure 3

Effects of HMGB1 administration and siRNA-mediated inhibition in HPAEpiC cells. (a) The relative expression levels of miR-205-5p and HMGB1 were determined by qPCR. The control and siRNA control (siRNA-NC) groups are indicated by black and green bars, respectively. (b) Results of a CCK8 proliferation assay. The results are presented as the means of three independent experiments. and vs. the other groups. (c) Western blot analysis of the levels of HMGB1, p65, p-IkBα/IkBα, and RAGE. Equivalent protein loading was verified using GAPDH or Lamin A as internal controls. (d) Representative micrographs of EdU-labeled samples from four groups and (e) corresponding fluorescence images of samples stained with Hoechst 33258. (f, h) Apoptotic cells were detected by flow cytometry and double-staining with Annexin V-FITC and PI. vs. the other groups. (g, i) Images of a flow cytometry-based cell cycle analysis of PI-labeled cells. and vs. the other groups. (j) ELISA analysis of the levels of HMGB1, IL-6, and TNF-α in culture supernatants. , , and vs. the other groups.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)