CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication

Figure 1

Generation and identification of sRNase L KO-PK cell line. (a) The plasmid map of Px459M and EZ-Guide-XH used to generate sRNase L KO-PK cell line. (b) The total RNAs of PK-15 and sRNase L KO-PK cells were extracted and analyzed by RT-PCR using PrimerScript One step RT-PCR Kit on 1% agarose gel. Lane 1, parental PK-15 cells (2232 bp band). Lane 2, sRNase L KO-PK cells (1392 bp). (c) RT-PCR product of the correct clone was sequenced and compared with the sRNase L gene sequence of PK-15 cells. (d) Western blotting analysis of sRNase L KO-PK cells. PK-15 and sRNase L KO-PK cells were seeded in 6-well plates for 16 h and harvested for Western blotting with anti-sRNase L antibody. The same blot was incubated by β-actin antibody as a protein loading control. The data showed here were results from one experiment of three Western blotting experiments.

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