CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication

Figure 3

Apoptosis in poly (I: C) transfected PK-15 and sRNase L KO-PK cells. (a) The nicked DNA in apoptotic cells was stained with green color by TUNEL assay and the nuclei were stained with blue color by DAPI. (b) The percentage of TUNEL-positive cells. The number of cells measuring of apoptosis was determined by counting 100 cells each in random microscopic fields. Each experiment was conducted in triplicate and repeated three times. Error bars indicate the standard deviations of three experiments. , P < 0.05.

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