CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication

Figure 4

RNase L reduces the transcript levels of ISGs. PK-15 and sRNase L KO-PK cells were transfected with poly (I: C). 7 h later, the transcript levels of ISG43, ISG15, ISG56, OAS1, OAS2, and Mx1 were analyzed by real-time RT-PCR. The cells without poly (I: C) treatment were used as negative controls and GAPDH was used as an internal control. The data represent the means of three independent experiments, with each experiment performed in triplicate. Data represent the means ± the standard deviations (error bars) of three experiments. , P < 0.05.