CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication

Figure 5

Growth kinetics of PRV in PK-15 and sRNase L KO-PK cells. PK-15 and sRNase L KO-PK cells were individually infected with PRV SD1404 or Bartha-K61 vaccine strain at an MOI of 1. Culture supernatants were collected at the indicated times. The virus titers of PRV SD1404 (a) or Bartha-K61 (c) strain were determined. The relative PRV DNA levels of PRV SD1404 (b) or Bartha-K61 (d) strain were analyzed by real-time PCR. The data represent the means of three independent experiments, with each experiment performed in triplicate. Error bars indicate the standard deviations of three experiments. , P < 0.05.

(a)

(b)

(c)

(d)