CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication

Table 1

Primers used in this study.

Primer name	Sequence (5’-3’)	Purpose
sRNase L-KO-3-Fwd	CACCTTCATGGAAGCCGCCGTGTA	sRNase L knockout
sRNase L-KO-3-Rev	AAACTACACGGCGGCTTCCATGAA
sRNase L-KO-4-Fwd	CACCCAGCCGAGCCAACGATAACG	sRNase L knockout
sRNase L-KO-4-Rev	AAACCGTTATCGTTGGCTCGGCTG
sRNase L-Fwd	ATGGAGACCAAGCGCCATAACAAC	sRNase L amplification
sRNase L-Rev	CTAGGTCTGGCCATCACCAGCTC
sISG43-Fwd	TGAGGAGCAGAGGAGAAATG	ISG43 amplification
sISG43-Rev	GGAGACAGTAGGCAAGTTCC
sISG15-Fwd	GCAATGTGCTTCAGGATGG	ISG15 amplification
sISG15-Rev	AGGCTTGAGGTCATACTCCC
sISG56-Fwd	GGAGTTGGTCATTCAAGACAC	ISG56 amplification
sISG56-Rev	CGTAAGGTAATACAGCCAGGC
sOAS1-Fwd	ATGCTGACCTCGTCGTCTTC	OAS1 amplification
sOAS1-Rev	GGACATCAAACTCCACCTCC
sOAS2-Fwd	TCTGGGCACAGTTGAAATG	OAS2 amplification
sOAS2-Rev	GATGCTCTGCTCTTTAGCG
sMx1-Fwd	TGAACGAAGAAGACGAATGG	Mx1 amplification
sMx1-Rev	CGTATGGCTGATTGCCTAC
sGAPDH-Fwd	ATCACCATCTTCCAGGAGC	GAPDH amplification
sGAPDH-Rev	TTCACGCCCATCACAAAC
PRV-Fwd	TGAACATCCTCACCGACTTC	PRV gD amplification
PRV-Rev	TAGAACGGCGTCAGGAATC