CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication
Table 2
Ability of sRNase L KO-PK and PK-15 cells for PRV propagation.
PRV strain
Harvest time
Virus titers in sRNase L KO-PK cells a
Virus titers in PK-15 cells a
Fold-difference in PRV titers b
SD1404
12 h
1×10 0.6±0.1 TCID50/mL
1×10 0.7±0.2 TCID50/mL
0.79
Bartha-K61
12 h
1×10 0.6±0.2 TCID50/mL
1×10 0.7±0.3 TCID50/mL
0.79
SD1404
24 h
1×10 3.2±0.1 TCID50/mL
1×10 3.0±0.3 TCID50/mL
1.58
Bartha-K61
24 h
1×10 3.1±0.3 TCID50/mL
1×10 2.9±0.1 TCID50/mL
1.58
SD1404
36 h
1×10 5.6±0.2 TCID50/mL
1×10 4.9±0.2 TCID50/mL
5.01
Bartha-K61
36 h
1×10 5.1±0.1 TCID50/mL
1×10 4.4±0.2 TCID50/mL
5.01
SD1404
48 h
1×10 6.9±0.2 TCID50/mL
1×10 6.2±0.1 TCID50/mL
5.01
Bartha-K61
48 h
1×10 6.4±0.2 TCID50/mL
1×10 5.6±0.3 TCID50/mL
6.31
SD1404
60 h
1×10 5.9±0.3 TCID50/mL
1×10 5.4±0.1 TCID50/mL
3.16
Bartha-K61
60 h
1×10 5.3±0.1 TCID50/mL
1×10 5.1±0.2 TCID50/mL
1.58
aData represent the means ± standard deviations of three independent experiments. ,P < 0.05. bFold-difference in PRV titers was obtained by PRV titers in sRNase L KO-PK cells/PRV titers in PK-15 cells.
