Cox-2 Negatively Affects the Protective Role of Propofol against Hypoxia/Reoxygenation Induced Cardiomyocytes Apoptosis through Suppressing Akt Signaling

Figure 4

Cox-2 suppressed Akt phosphorylation negatively affects the protective effect of propofol against hypoxia/reoxygenation induced cardiomyocyte apoptosis. (a) Western blot analysis of cleaved Caspase-3, total Caspase-3, Akt and p-Akt under indicated conditions. (b) Expression of p-Akt (Thr450) under indicated conditions. (c) Western blot analysis of Akt and p-Akt under indicated conditions. NC means negative control; OE means over-expression; HR means H/R treatment group; P means treatment of propofol; Cox-2 means Cox-2 over-expression; SC79 means treatment of SC79 (10 μM). Data were presented as the mean ± SD (n ⩾ 3). P<0.05, P<0.01.

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