Stable Reference Gene Selection for RT-qPCR Analysis in Synechococcus elongatus PCC 7942 under Abiotic Stresses

Table 1

Primer sequences and amplicon characteristics in the candidate reference genes.

Gene symbol	CYORF ID	Definition	Primer sequence (5′→3′)	Amplicon	Tm	E (%)	R^2	Median Ct
			(Forward/Reverse)	length (bp)	(°C)
16S	Synpcc 7942_R0052	16S ribosomal RNA	F: GCAAGCCTGACGGAGCAAC	180	58	96.3	0.997	9.13
			R: CGGACGCTTTACGCCCAAT
petB	Synpcc 7942_2331	cytochrome b6	F: CTGATCCGCTCCATCCACC	296	58	90.4	0.999	20.88
			R: GTTGCCTGACCGACGCTTT
ppc	Synpcc 7942_2252	phosphoenolpyruvate carboxylase	F: CCCTTGCCAGGACCAGATGA	219	62	96.7	0.999	25.83
			R: CGTCGGGTGAGCGGTGAAAA
ilvD	Synpcc 7942_0626	dihydroxy-acid dehydratase	F: CGGCGCTGCGGCTCAATAT	128	58	97.4	0.999	24.09
			R: ATCATCGGCGGCGACAACC
prs	Synpcc 7942_2113	ribose-phosphate pyrophosphokinase	F: TCTTGCCCTACTACGGTTACGC	102	58	91.1	0.999	24.70
			R: TCGCTCCAGCCTGAGTGATTAG
rnpA	Synpcc 7942_1615	ribonuclease P protein component	F: CTCGTTGCCATCGACTGCG	130	62	94	0.996	26.93
			R: CGACTTTCGTTGACGGACTCTATTT
rnpB	Synpcc 7942_R0036	RNA component of RNaseP	F: TACCGCCGATGGCCTGCTT	119	58	105.1	0.998	16.15
			R: TGTCCCTCCACCTTGCTCCC
secA	Synpcc 7942_0289	preprotein translocase, SecA subunit	F: ACGACGGTCAGATTGCCGAGAT	204	59.1	97.6	0.998	24.97
			R: GCGACATTCCCTGCTGGATTAG
rimM	Synpcc 7942_2259	16S rRNA processing protein RimM	F: TGATTGACCAAGCCAGCAGCAC	121	62	96.2	0.997	24.88
			R: GCCTCCACAAAGGGAATGTAGACC