Stable Reference Gene Selection for RT-qPCR Analysis in Synechococcus elongatus PCC 7942 under Abiotic Stresses

Table 3

Ranking of the candidate reference genes via the Pearson’s correlation coefficient (r) and probability values (p), as calculated by BestKeeper software.

Rank	ALL			NTC			NaCL			HO			HL
	Gene	r	p-value	Gene	r	p-value	Gene	r	p-value	Gene	r	p-value	Gene	r	p-value
1	secA	0.966	0.001	16S	0.937	0.001	petB	0.905	0.001	rnpA	0.972	0.001	petB	0.987	0.001
2	prs	0.956	0.001	secA	0.92	0.001	rnpA	0.885	0.001	16S	0.968	0.001	prs	0.983	0.001
3	16S	0.951	0.001	rnpA	0.881	0.001	rimM	0.882	0.001	secA	0.954	0.001	rnpA	0.976	0.001
4	petB	0.929	0.001	ppc	0.878	0.001	16S	0.87	0.001	ilvD	0.946	0.001	secA	0.968	0.001
5	rnpA	0.922	0.001	prs	0.862	0.001	prs	0.858	0.001	ppc	0.939	0.001	rimM	0.964	0.001
6	ilvD	0.855	0.001	ilvD	0.836	0.001	ilvD	0.857	0.001	petB	0.935	0.001	ppc	0.958	0.001
7	ppc	0.762	0.001	petB	0.721	0.005	ppc	0.811	0.001	prs	0.926	0.001	ilvD	0.935	0.001
8	rimM	0.681	0.001	rnpB	0.57	0.042	secA	0.669	0.012	rnpB	0.749	0.003	16S	0.924	0.001
9	rnpB	0.543	0.001	rimM	0.331	0.271	rnpB	0.477	0.099	rimM	0.506	0.077	rnpB	0.745	0.004