Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA

Figure 6

Effects of IMP on activation of NF-κB signaling pathway in RBL-2H3 cells. RBL-2H3 cells were divided into the Con, IgE+Ag, and IgE+Ag+IMP groups. RBL-2H3 cells were stimulated with 10 μg/ml anti-DNP IgE for 6 h and challenged with 100 ng/ml DNP-HAS, in the absence or presence of IMP (10, 20, and 40 μM, respectively). The key proteins of NF-κB signaling pathway were detected by Western blot analysis. Compared with the Con group, ^∗P<0.05, ^∗∗P<0.01; compared with the IgE+Ag group, ^#P<0.05, ^##P<0.01.