Linking De Novo Assembly Results with Long DNA Reads Using the dnaasm-link Application

Figure 5

Comparison of calculation time and peak of RAM memory usage of the SSPACE-LongReads, LINKS and dnaasm-link applications. The experiment was conducted out on the Caenorhabditis elegans genome (GenBank NC_003279 … NC_003284, NC_001328). Firstly, a set of eleven subgenomes of sizes 1Mbp, 10Mbp, 20Mbp … 100Mbp was generated from genome. Then, for each sequence a set of long and short DNA reads was generated. Short DNA reads were de novo assembled by ABySS. Finally, the set of resulting contigs and long DNA reads were used as input data sets in the presented experiment.