Wnt Signaling Protects against Paclitaxel-Induced Spiral Ganglion Neuron Damage in the Mouse Cochlea In Vitro

Figure 4

Inhibition and activation of Wnt signaling affected β-catenin and C-myc expression in SGNs after paclitaxel treatment. (a) The diagram of the assay for (b)–(f). The middle turn cochlear SGN explants from P3 mice were cultured in vitro, and the explants were treated with 30 μM paclitaxel, 30 μM paclitaxel together with a Wnt agonist (10 μg/ml RS-1), or 30 μM paclitaxel together with a Wnt antagonist (10 μM IWP-2) for 48 h and then used for immunostaining and qPCR. (b–e) Immunofluorescence revealed the treatment with RS-1 resulted in an increase in the protein expression of β-catenin and C-myc in SGNs, while treatment with IWP-2 markedly reduced the expression of β-catenin and C-myc in SGNs compared with the paclitaxel-only group. (f) qPCR showed that the mRNA expression of β-catenin and C-myc was significantly upregulated after treatment with RS-1. , .

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