Wnt Signaling Protects against Paclitaxel-Induced Spiral Ganglion Neuron Damage in the Mouse Cochlea In Vitro

Figure 6

Wnt signaling regulated the caspase-mediated apoptosis in SGNs as a result of paclitaxel treatment. (a) The diagram of the assay for (b)–(e). The cochlear SGN explants from P3 mice were treated with 30 μM paclitaxel, 30 μM paclitaxel together with a Wnt agonist (10 μg/ml RS-1), or 30 μM paclitaxel together with a Wnt antagonist (10 μM IWP-2) for 48 h and then used for immunostaining. (b–e) After paclitaxel treatment, the SGN explants treated with paclitaxel and RS-1 had significantly fewer TUNEL/Tuj 1 and cleaved-caspase 3/Tuj 1 double-positive SGNs compared to the paclitaxel-only group, whereas the paclitaxel and IWP-2 cotreated cochleae had significantly more TUNEL/Tuj 1 and cleaved-caspase 3/Tuj 1 double-positive SGNs. , .

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