Histological Analysis of Bone Repair in Mandibular Body Osteotomy Using Internal Fixation System in Three Different Gaps without Bone Graft in an Animal Model

Figure 3

Bone repair of rabbit mandible (Oryctolagus cuniculus) after an osteotomy with 5 mm separation between the segments and internal rigid fixation system 1.5. (a) Cartilaginous callus on the osteotomy line (HE). (b) Area peripheral to the callus shows trabeculae in formation whose cartilaginous components have been replaced by bone tissue by means of endochondral ossification (HE). (c) and (d) In the cartilaginous callus, active chondrocytes immersed in a matrix positive to Alcian blue staining (HE, Alcian blue, pH 2.5) are observed. (e) Cancellous bone tissue next to the callus shows wide medullary spaces with abundant fibroblasts and blood vessels (Van Gieson). (f) Type III collagen (green) mainly in the cartilaginous tissue matrix and in the trabeculae peripheral to the callus (Picrosirius red). (g) and (h) Peripheral fibrous reaction, without inflammatory elements in areas of contact with the screws (Van Gieson).