Research Article
Optimizing Culture Conditions by Statistical Approach to Enhance Production of Pectinase from Bacillus sp. Y1
Table 4
Experimental design and pectinase activities (U/mL) of the central composite design.
| Run no. | Experimental factors and code levels | PGL | PG | Carbon source | (NH4)2SO4 | Initial pH | Experiment | Predicted | Experiment | Predicted | (%, v/w) | (%, v/w) |
| 1 | –1 (9.00) | +1 (0.45) | –1 (7.50) | 22.1 ± 0.6 | 23.8 | 31.9 ± 0.8 | 32.3 | 2 | –1.68 (8.32) | 0 (0.30) | 0 (8.50) | 23.2 ± 0.9 | 23.3 | 31.7 ± 1.0 | 32.0 | 3 | 0 (10.00) | 0 (0.30) | 0 (8.50) | 26.9 ± 1.1 | 26.9 | 33.8 ± 1.4 | 33.7 | 4 | +1 (11.00) | +1 (0.45) | +1 (9.50) | 21.8 ± 0.7 | 23.4 | 34.7 ± 0.9 | 34.5 | 5 | 0 (10.00) | 0 (0.30) | 0 (8.50) | 26.9 ± 1.1 | 26.9 | 33.8 ± 1.4 | 33.7 | 6 | 0 (10.00) | 0 (0.30) | 0 (8.50) | 26.9 ± 1.1 | 26.9 | 33.8 ± 1.4 | 33.7 | 7 | 0 (10.00) | 0 (0.30) | 0 (8.50) | 26.9 ± 1.1 | 26.9 | 33.8 ± 1.4 | 33.7 | 8 | 0 (10.00) | –1.68 (0.05) | 0 (8.50) | 6.5 ± 0.3 | 9.0 | 17.7 ± 1.0 | 18.7 | 9 | 0 (10.00) | 0 (0.30) | –1.68 (6.82) | 23.2 ± 1.0 | 23.8 | 31.6 ± 0.9 | 31.5 | 10 | +1.68 (11.68) | 0 (0.30) | 0 (8.50) | 24.6 ± 0.5 | 25.3 | 35.3 ± 0.4 | 35.9 | 11 | 0 (10.00) | 0 (0.30) | 0 (8.50) | 26.9 ± 1.1 | 26.9 | 33.8 ± 1.4 | 33.7 | 12 | 0 (10.00) | +1.68 (0.55) | 0 (8.50) | 26.0 ± 0.5 | 24.2 | 32.1 ± 1.1 | 32.0 | 13 | +1 (11.00) | +1 (0.45) | –1 (7.50) | 24.8 ± 0.8 | 24.1 | 34.7 ± 0.7 | 34.5 | 14 | +1 (11.00) | –1 (0.15) | +1 (9.50) | 13.3 ± 0.6 | 11.0 | 22.4 ± 1.1 | 21.3 | 15 | +1 (11.00) | –1 (0.15) | –1 (7.50) | 23.2 ± 0.9 | 22.9 | 28.3 ± 0.6 | 28.2 | 16 | –1 (9.00) | +1 (0.45) | +1 (9.50) | 26.0 ± 0.9 | 25.8 | 28.9 ± 0.8 | 28.4 | 17 | 0 (10.00) | 0 (0.30) | 0 (8.50) | 26.9 ± 1.1 | 26.9 | 33.8 ± 1.4 | 33.7 | 18 | 0 (10.00) | 0 (0.30) | +1.68 (10.18) | 15.2 ± 0.6 | 15.4 | 21.4 ± 1.2 | 22.5 | 19 | –1 (9.00) | –1 (0.15) | +1 (9.50) | 8.7 ± 0.3 | 8.9 | 19.4 ± 0.3 | 18.9 | 20 | –1 (9.00) | –1 (0.15) | –1 (7.50) | 20.3 ± 0.4 | 18.1 | 30.1 ± 0.6 | 29.7 |
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All figures in parenthesis are the actual value of factors in the experiments. |