Dysbiosis and Variation in Predicted Functions of the Granulation Tissue Microbiome in HPV Positive and Negative Severe Chronic Periodontitis

Table 1

Summary of the primer pair used to generate the 16S rDNA gene fragment fragments and the characteristics of each region in CP samples.

Region		Forward Primers (with forward barcode-Red)	Amplicon length (Without Barcode)
V1-V9	F	AGRGTTYGATYMTGGCTCAG	1,464
	F-1	TGAGTGACGTGTAGCGAGRGTTYGATYMTGGCTCAG
	F-2	GACAGCATCTGCGCTCAGRGTTYGATYMTGGCTCAG
	F-3	TGCGAGCGACTCTATCAGRGTTYGATYMTGGCTCAG
	F-4	TGCTCTCGTGTACTGTAGRGTTYGATYMTGGCTCAG
Region		Reverse Primers (with reverse barcode-Red)
V1-V9	R	RGYTACCTTGTTACGACTT	1,464
	R-1	GCTCGACTGTGAGAGA RGYTACCTTGTTACGACTT
	R-2	TGCTCGCAGTATCACA RGYTACCTTGTTACGACTT
	R-3	GCAGACTCTCACACGC RGYTACCTTGTTACGACTT
	R-4	AGACAGCATCTGCGCTCRGYTACCTTGTTACGACTT

where
F= Forward primer,
F-1= Forward barcode primer 1,
F-2= Forward barcode primer 2,
F-3= Forward barcode primer 3,
F-4= Forward barcode primer 4
R= Reverse Primer,
R-1= Forward barcode primer 1,
R-2= Forward barcode primer 2,
R-3= Forward barcode primer 3,
4-4= Forward barcode primer 4,
R = A or G,
Y = C or T,
M = A or C, and
N = any base.