Vitamin D Receptor Gene Polymorphisms and Autoimmune Thyroiditis: Are They Associated with Disease Occurrence and Its Features?

Table 2

PCR conditions for genotyping of rs2228570 (FokI), rs1544410 (BsmI), rs7975232 (ApaI), and rs731236 (TaqI) VDR SNPs.

SNP	Forward primer	Reverse primer	PCR conditions	Cycles	PCR product size (bp)	Enzyme	Digested products size (bp)
rs2228570	5’-AGCTGGCCCTGGCACTGACTCTGGCT-3’	5’-ATGGAAACACCTTGCTTCTTCTCCCTC-3’	95°C for 5 min; (95°C for 30 s; 63°C for 30 s; 72°C for 30 s); 72°C for 7 min	33	267	FokI	CC: 267; CT: 267, 197, and 70; TT: 197 and 70
rs1544410	5’-ACTTGCATGAGGAGGAGCATGTC-3’	5’-GGAGAGGAGCCTGTGTCCCATTTG-3’	95°C for 5 min; (95°C for 45 s; 61°C for 30 s; 72°C for 45 s); 72°C for 7 min	36	812	BsmI	GG: 475 and 337; GA: 812, 475, and 337; AA: 812
rs7975232 / rs731236	5’-GGTGGGATTGAGCAGTG-3’	5’-ATGCTGCACTCAGGCTG-3’	95°C for 5 min; (95°C for 30 s; 56°C for 30 s; 72°C for 45 s); 72°C for 7 min	35	280	ApaI / TaqI	ApaI: CC: 256 and 24; CA: 280, 256, and 24; AA: 280; TaqI: TT: 280; TC: 180 and 100; CC: 280, 180, and 100

SNP, single nucleotide polymorphism; bp, base pair.