BioMed Research International / 2019 / Article / Tab 2

Research Article

Vitamin D Receptor Gene Polymorphisms and Autoimmune Thyroiditis: Are They Associated with Disease Occurrence and Its Features?

Table 2

PCR conditions for genotyping of rs2228570 (FokI), rs1544410 (BsmI), rs7975232 (ApaI), and rs731236 (TaqI) VDR SNPs.

SNPForward primerReverse primerPCR conditionsCyclesPCR product size (bp)EnzymeDigested products size (bp)

rs22285705’-AGCTGGCCCTGGCACTGACTCTGGCT-3’5’-ATGGAAACACCTTGCTTCTTCTCCCTC-3’95°C for 5 min; (95°C for 30 s; 63°C for 30 s; 72°C for 30 s); 72°C for 7 min33267FokICC: 267 
CT: 267, 197, and 70 
TT: 197 and 70

rs15444105’-ACTTGCATGAGGAGGAGCATGTC-3’5’-GGAGAGGAGCCTGTGTCCCATTTG-3’95°C for 5 min; (95°C for 45 s; 61°C for 30 s; 72°C for 45 s); 72°C for 7 min36812BsmIGG: 475 and 337 
GA: 812, 475, and 337 
AA: 812

rs7975232 / rs7312365’-GGTGGGATTGAGCAGTG-3’5’-ATGCTGCACTCAGGCTG-3’95°C for 5 min; (95°C for 30 s; 56°C for 30 s; 72°C for 45 s); 72°C for 7 min35280ApaI / TaqIApaI:  
CC: 256 and 24 
CA: 280, 256, and 24 
AA: 280 
TT: 280 
TC: 180 and 100 
CC: 280, 180, and 100

SNP, single nucleotide polymorphism; bp, base pair.

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