The Differential Expression of miRNAs and a Preliminary Study on the Mechanism of miR-194-3p in Keloids

Figure 1

The expression of miRNA-194-3p is downregulated, and RUNX2 can be regulated. (a) Heat map of the microarray analysis. (b) As detected by real-time PCR, miR-194-3p has lower expression in keloids compared with normal skin, P< 0.05. (c) The target binding site between miRNA-194-3p and RUNX2 was detected using miRDB and targetscan software. (d) The luciferase reporter gene experiment showed that miR-194-3p regulates the activity of RUNX2 in both primary cultured fibroblasts and HKF cells, P< 0.05. (e) Western blot analysis showed that the expression of RUNX2 was inhibited after the overexpression of miR-194-3p. (2C) Real-time PCR showed that the expression of RUNX2 mRNA was inhibited after the overexpression of miR-194-3p. (f) The expression level of RUNX2 increased after the inhibition of miR-194-3p. (g) When miR-194-3p was suppressed, the level of RUNX2 mRNA increased.

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