The Differential Expression of miRNAs and a Preliminary Study on the Mechanism of miR-194-3p in Keloids

Figure 3

Effect of miR-194-3p on the migration of fibroblasts. (a) When miR-194-3p was overexpressed, the migration of fibroblasts and HKF cells decreased, P< 0.05. (b) When miR-194-3p was inhibited, the migration of fibroblasts and HKF was observed, P< 0.05. (c) Wound scratch assay was performed to analyze cell migration. miR-194-3p overexpression inhibited HKF migration, while miR-194-3p inhibitor promoted HKF migration. (d, e) Western blot analysis and real-time PCR showed that the expression of MMP2 was inhibited after miR-194-3p was overexpressed, P< 0.05. (f, g) Western blot analysis and real-time PCR showed that the expression of MMP2 increased after the inhibition of miR-194-3p, P< 0.05.

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