The Differential Expression of miRNAs and a Preliminary Study on the Mechanism of miR-194-3p in Keloids

Figure 4

RUNX2 is crucial to the proliferation and migration of HKF cells (a) Effect of miR-194-3p siRNA and RUNX2 siRNA on cell proliferation as measured by MTT assay. Data are presented as the mean ± SEM. P< 0.05 vs. miR-194-3p inhibitor group, P< 0.05 vs. si-RUNX2 group, P< 0.05 miR-194-3p inhibitor group vs. si-RUNX2+miR-194-3p inhibitor group, and ## P< 0.05 si-RUNX2 group vs. si-RUNX2+miR-194-3p inhibitor group. (b) Effect of miR-194-3p siRNA and RUNX2 siRNA on cell migration ability, detected with the transwell assay. Cells were counted, and results represent the mean ± SD of three experiments. ×200 P< 0.05 vs. miR-194-3p inhibitor group, P< 0.05 vs. si-RUNX2 group, P< 0.05 miR-194-3p inhibitor group vs. si-RUNX2+miR-194-3p inhibitor group, and ## P< 0.05 si-RUNX2 group vs. si-RUNX2+miR-194-3p inhibitor group. (c, d) The effects of miR-194-3p siRNA and RUNX2 siRNA on RUNX2, Cyclin D1, and MMP2 were detected by western blotting and real-time PCR. Data are shown as the mean ± SEM. P< 0.05 vs. miR-194-3p inhibitor group, P< 0.05 vs. si-RUNX2 group, P< 0.05 miR-194-3p inhibitor group vs. si-RUNX2+miR-194-3p inhibitor group, and ## P< 0.05 si-RUNX2 group vs. si-RUNX2+miR-194-3p inhibitor group.

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