Laboratory and Clinical Evaluation of DNA Microarray for the Detection of Carbapenemase Genes in Gram-Negative Bacteria from Hospitalized Patients

Table 1

The primer sequences for microarray.

Primer	Sequence (5′-3′)	Positions	Targeted gene/; GenBank accession
KPC-F	CTGGGCAGTCGGAGACAAAA	681-700	KPC/ KX619622.1
KPC-R	AGACGGCCAACACAATAGGT	765-784
NDM1-F	GAATGTCTGGCAGCACACT	168-186	NDM-1/ KX249707
NDM1-R	TGGCATAAGTCGCAATCC	407-424
OXA23-F	GCAGTCCCAGTCTATCAGGA	379-398	OXA-23/NG_049726
OXA23-R	CCCAACCAGTCTTTCCAA	641-658
OXA48-F	TCGGGCAATGTAGACAGTT	548-556	OXA-48/ NG_049762
OXA48-R	CACCAGCCAATCTTAGGTTC	746-765
OXA51-F	GCTCGTCGTATTGGACTTGA	406-425	OXA-51/ KX609247
OXA51-R	TGTGCCTCTTGCTGAGG	523-539
IMP-F	GTAATTGACACTCCATTTAC	291-309	IMP /NG_049172
IMP-R	GCGGACTTTGGCCAAGCTTC	674-693
VIM-F	TGGTGAGTATCCGACAG	190-206	VIM/NG_050336
VIM-R	ATGAAAGTGCGTGGAG	433-448
DIM-F	GCTTGTCTTCGCTTGCTAA	38-56	DIM/NG_049077
DIM-R	ATTCCTGCGGTTCTATCCT	293-311
mtDNA-F	GTCGAAGGTGGATTTAGCAGTAA	1413-1435	mtDNA/MG182040
mtDNA-R	GTAAGGTGGAGTGGGTTTGGG	1684-1704
182-F	AGAGTTTGATCMTGGCTCAG	1-20	16S rRNA/ LN612729
756-R	CGTATTACCGCGGCTGCTG	518-530

F, forward primer; R, reverse primer; all reverse primers have biotin conjugated at 5′-ends.
Positions refer to the nucleotide numberings of the corresponding GenBank genes.