Laboratory and Clinical Evaluation of DNA Microarray for the Detection of Carbapenemase Genes in Gram-Negative Bacteria from Hospitalized Patients

Table 2

The probe sequences for microarray.

Probe	Sequences (5′-3′)	Targeted gene
KPC-P	CAAATGACTATGCCGTCGTCTGGCC	KPC
NDM1-P1	ACCGATGACCAGACCGCCCAGATCCTCAAC	NDM-1
NDM1-P2	TCAGGACAAGATGGGCGGTATGGAC	NDM-1
OXA23-P1	TTTTAGAAGAGAGTAATGGCTACAAAA	OXA-23
OXA23-P2	ATTGGACAGCAGGTTGATAATTTCTGG	OXA-23
OXA48-P1	CGAATTTCGGCCACGGAGCAAATCAGCTT	OXA-48
OXA48-P2	CAGCGTATTGTCAAACAAGCCATGC	OXA-48
OXA51-P1	GAAGTGAAGCGTGTTGGTTATG	OXA-51
OXA51-P2	ATATCGGTACCCAAGTCGATAATTTTTGGC	OXA-51
IMP-P1	GGCTAGTTAAAAATAAAATTGAAG	IMP
IMP-P2	CCCACGTATGCRTCTGAATTAAC	IMP
VIM-P1	TGGTGTTTGGTCGCATATCGCAACG	VIM
VIM-P2	CTCATTGTCCGTGATGGTGATGAG	VIM
DIM-P1	GTCAGTTCAAACGGCCTTGTTGTCATAGATT	DIM
DIM-P2	CTTGGTCAGACCGAGATACAGAAACGCTCG	DIM
mtDNA-P	ATGTCCTTTGAAGTATACTTGAGGAGTT	mitochondria
551-P	ACTCCTACGGGAGGCAGCAGTT	16S rRNA
Quality control	TTTTTTTTTTTTTTTTTTTT	Oligo dT20

An oligonucleotide of 12 T’s with an amino-labeled 3′-end was conjugated to the 3′-ends of all probes. An oligonucleotide of 20 T’s with an amino-labeled 3′-end, biotin-labeled 5′-end was used as microarray quality control.