Phenothiazinium Dyes Are Active against Trypanosoma cruzi In Vitro

Table 1

In vitro and toxicity of MB, NMB, TBO, and DMMB to T. cruzi amastigotes and LLCMK2 cells. The molecular weight (MW), , , and selectivity index (SI) were calculated for MB, NMB, TBO, DMMB, and BZ on T. cruzi and LLCMK2 cells. Amastigotes or LLCMK2 cells were incubated for 72 hours, at 37°C, with 5% CO₂, and the proliferation (amastigotes) or toxicity (LLCMK2 cells) was measured after CRPG or MTT assays, respectively. The percentage of inhibition was calculated in comparison to the nontreated controls in three independent assays.

Compound		MW	μM	μM	SI
MB		319.85	0.44 ± 0.15	16.82 ± 1.18	37.40
NMB		347.91	0.09 ± 0.01	4.19 ± 1.30	44.71
TBO		305.83	0.27 ± 0.11	7.93 ± 5.22	28.61
DMMB		416.05	0.08 ± 0.02	4.46 ± 0.23	51.26
BZ		260.25	3.41 ± 0.38	> 200	> 58.65