PEGylated Polyethylenimine Derivative-Mediated Local Delivery of the shSmad3 Inhibits Intimal Thickening after Vascular Injury
Figure 2
Characterization of PEI-Et/shSmad3 and PEG-Et 1:1/shSmad3 complexes. (a) Agarose gel electrophoresis of PEI-Et/shSmad3 complexes at various N/P ratios. (b) Agarose gel electrophoresis of PEG-Et 1:1/shSmad3 complexes at various N/P ratios. Lane 1: Marker, Lane 2: naked shSmad3, and Lanes 3-7: polymer/shSmad3 complexes at N/P ratios of 1, 3, 5, 10, and 20. (c) Particle size and zeta potential of PEI-Et/shSmad3 complexes at various N/P ratios. (d) Particle size and zeta potential of PEG-Et 1:1/shSmad3 complexes at various N/P ratios. (e) Representative transmission electron microscopy image of PEI-Et/shSmad3 complexes at N/P ratio of 20. (f) Representative transmission electron microscopy image of PEG-Et 1:1/shSmad3 complexes at N/P ratio of 20.