BioMed Research International / 2019 / Article / Fig 3

Research Article

PEGylated Polyethylenimine Derivative-Mediated Local Delivery of the shSmad3 Inhibits Intimal Thickening after Vascular Injury

Figure 3

Cytotoxicity and gene transfection efficiency analysis of the PEG-Et 1:1. (a) Cytotoxicity analysis of the PEI 25 kDa, PEI-Et, and PEG-Et 1:1 at various concentrations (1, 5, 10, 20, 50, and 100 μg/ml) in VSMC cell lines. (b) Cytotoxicity analysis of PEG-Et 1:1/scrambled shRNA complexes at various N/P ratios (N/P ratio: 3, 5, 10, 20, 30, and 50) in VSMCs cell lines. (c) Fluorescence images of negative control group (N) and PEI 25 kDa/shSmad3 group (P). (d) Flow cytometry analyzed graphs of negative control group (N) and PEI 25 kDa/shSmad3 group) (P). (e) Fluorescence images of PEI-Et/shSmad3 group at w/w ratios of 3, 5, 10, 20, 30, and 50. (f) Flow cytometry analyzed graphs of PEI-Et/shSmad3 group at w/w ratios of 3, 5, 10, 20, 30, and 50. (g) Fluorescence images of PEG-Et 1:1/shSmad3 group at w/w ratios of 3, 5, 10, 20, 30, and 50. (h) Flow cytometry analyzed graphs of PEG-Et 1:1/shSmad3 group at w/w ratios of 3, 5, 10, 20, 30, and 50.

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