Positive Correlation between Activated CypA/CD147 Signaling and MMP-9 Expression in Mice Inflammatory Periapical Lesion

Table 1

Numbers of CypA⁺ Cells, CD147⁺ cells, and MMP-9⁺cells per high-power field ( 400) and percentage of osteoclasts, bone resorption volumes, and surface of periapical lesions (mean ± standard deviation).

GROUP	N	CypA^+ cells/hpf	CD147^+ cells/hpf^ᴥ	TRAP^+ cells/hpf^ԓ	MMP-9^+ cells/hpf^⨲	Lesion Volume (mm^3)	Lesion Surface (mm^2)^ф
Day 0	5	1.67 ± 2.08^§фɣǂ	--^‖§фɣǂ†	0.14 ± 0.38^§фɣǂϮ	--^‖§фɣǂϮ	0.063 ± 0.012^фɣǂϮ	0.022 ± 0.011^фɣǂϮ
Day 7	5	3.75 ± 1.26^§фɣǂ	5.67 ± 1.54	4.17 ± 1.72^§фɣǂ	3.67 ± 0.58^‖§фɣǂϮ	0.088 ± 0.038^ɣǂϮ	0.023 ± 0.009^фɣǂϮ
Day 14	5	7.17 ± 2.31^§фɣǂ	6.5 ± 1.29^†	7.22 ± 2.60^фɣ	24.6 ± 4.83^‡†фɣ	0.133 ± 0.045^ɣǂϮ	0.031 ± 0.007^ɣǂϮ
Day 21	5	11.16 ± 3.25^†‡ф‖	9.33 ± 2.58^†	9.88 ± 2.03^†‡	27.25 ± 6.85^‡†ɣ	0.157 ± 0.067^ɣǂϮ	0.035 ± 0.006^ɣǂϮ
Day 28	5	15.56 ± 8.59^†‡‖§ɣǂ	11.25 ± 1.83^†Ϯ	11.71 ± 2.21^†‡	34.13 ± 8.22^‡†‖ǂϮ	0.257 ± 0.064^†	0.048 ± 0.013^†‡
Day 35	5	10.78 ± 2.22^†‡‖ф	10.1 ± 2.30^†Ϯ	13.8 ± 3.03^†‡	44.67 ± 10.28^‖§ǂϮ†‡	0.316 ± 0.086^†‡‖§	0.049 ± 0.008^†‡‖§
Day 42	5	13 ± 2.65^†‡‖ф	6.67 ± 0.58^†	8.67 ± 1.63^†‡	25.57 ± 4.12^‡ɣ†ф	0.351 ± 0.043^†‡‖§	0.055 ± 0.005^†‡‖§
Day 49	5	7 ± 2^†фǂ	4.67 ± 0.58^†фϮ	8 ± 2.35^†	24 ± 5.29^‡ɣ†ф	0.349 ± 0.014^†‡‖§	0.058 ± 0.004^†‡‖§

The greatest numbers of CypA +cells and CD147+ cells per high-power field appeared on day 28. The greatest percentage of MMP-9+ cells and osteoclasts reached on day 35. The greatest lesion volume appeared on day 42 and the greatest lesion surface appeared on day 49. Significant differences were found between day 0 and days 21, 28, 35, 42, and 49 and between day 7 and day 28 values of CypA+ cells. Statistical significance between groups was showed by tags. Positive correlation was found between lesion volume and surface and between numbers of CypA+ cells, CD147+ cells, MMP-9+ cells, and osteoclasts. hpf, high-power field.
^ԓPositive correlation (R² = 0.5101, P < .01) with number of CypA + cells/hpf.
^ᴥPositive correlation (R² =0.4423, P < .01) with number of CypA + cells/hpf.
^⨲ Positive correlation (R² = 0.4715, P < .01) with number of CypA + cells/hpf.
^фPositive correlation (R² = 0.4053, P < .01) with lesion volume.
^‡P < .05 versus 7-day group.
^‖P < .05 versus 14-day group.
^§P < .05 versus 21-day group.
^фP < .05 versus 28-day group.
^ɣP < .05 versus 35-day group.
^ǂP < .05 versus 42-day group.
^ϮP < .05 versus 49-day group.
^†P < .05 versus 0-day group (control).