Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs

Figure 1

Generation and characterization of the recombinant rAd-PEDV-S virus. (a) Measurement of expression levels of the recombinant virus rAd-PEDV-S in infected HEK293A cells. Noninfected HEK293A cells were used as a negative control. (b) Western blot demonstrating the expression of full-length PEDV S (approximately 153 kDa) by the recombinant virus rAd-PEDV-S in the cell culture in vitro. Cell lysates from cells infected with wild-type adenovirus were used as negative controls. The blot was developed with an anti-His-tag mAb. (c) Titer of the recombinant virus determined by the rAd-PEDV-S fluorescence dilution assay. Green fluorescence was emitted by the enhanced green fluorescent protein (EGFP) derived from the vector. The results showed that the recombinant virus rAd-PEDV-S could be well replicated in HEK293A cells and that its titers could reach 10¹¹ PFU/ml.

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