Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs

Table 2

Summary of clinical scores and fecal viral shedding for the challenged piglets in each group.

Dpi	Ad-blank^a (n = 5)			rAd-PEDV-S^a (n = 5)			Inactivated vaccine^a (n = 5)			PBS^a (n = 5)
	NP	CT	CS	NP	CT	CS	NP	CT	CS	NP	CT	CS
0	0/5	—	0	0/5	—	0	0/5	—	0	0/5	—	0
1	1/5	25.67	2	0/5	—	0	0/5	—	0	2/5	23.42–26.79	3
2	3/5	22.36–23.82	2‐3	0/5	—	0	0/5	—	0	5/5	22.73–25.18	3
3	5/5	21.58–24.12	2‐3	0/5	—	0	0/5	—	0
4				1/5	23.02	2	1/5	25.44	1
5				2/5	21.52–26.26	2	2/5	24.58–26.43	3
6				3/5	23.54–28.09	2‐3	2/5	23.69–26.87	2
7				3/5	24.27–25.59	2‐3	2/5	25.80–28.99	2‐3

^aPigs were challenged orally with 2 mL of 1 × 10⁵ TCID₅₀ highly virulent genotype GIIb PEDV strain. Dpi: days post inoculation for pigs; NP: number of PEDV-positive piglets; CT: cycle threshold value—a value greater than 30 was considered negative or below the detection limit of real-time PCR; CS: clinical score for fecal consistency—0 = normal, 1 = pasty, 2 = semiliquid, and 3 = liquid; —: samples with no CT value (no PEDV RNA detected). The blocks without any entry indicate that the PEDV-positive piglets were euthanized after infection was confirmed by clinical symptoms and fecal viral shedding.