Inhibitory Effect of Cuphea aequipetala Extracts on Murine B16F10 Melanoma In Vitro and In Vivo
Figure 6
Induction of apoptosis by Cuphea aequipetala extract in B16F10 cells. (a) Representative plots of B16F10 cells without treatment (Control) and exposed to methanolic extract (CC50 [0.269 mg/mL]) and etoposide (150μM); in the figure the quadrant distribution (double negative in lower left quadrant, Annexin-V-APC+ in lower right quadrant, double positive in upper right as and upper left quadrant as PI+) is observed. (b) DNA fractionation assay. Lane 1 corresponds to B10F10 cells without treatment, and lane 2 cells treated with methanolic extract. (c) Caspase-3 activity test. Included cells without treatment (Control) and cells treated with methanolic extract. Bar graphs represent the mean (±SD) of triplicates of at least three independent experiments. p < 0.05 (), p < 0.01 (), and p < 0.001 ().