Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity
Figure 2
Effects of various preservation temperatures on endothelial cells viability and on the mitochondria. (a) LDH release, measured at the end of the hypoxic preservation, is expressed as LDH release in supernatant/ intracellular LDH (U/L). Results are expressed in mean ± SEM (n = 8 per group). CTL: control cells not subjected to any manipulation. The integrity and activity levels of mitochondrial respiratory chain complexes were measured after normothermic reoxygenation: (b) complex II (n = 16 per group); (c) ATP production by Complex V (n = 16 per group). Results are expressed as mean ± SEM (% of control cells). Statistical significance (p<0.05) were calculated using nonparametric Kruskal-Wallis test, followed by multiple comparison evaluation by Dunn’s posttest (§ p<0.05 to control cells, p<0.05 versus 4°C, † p<0.05 versus 19°C, £ p<0.05 versus 27°C, ‡ p<0.05 versus 37°C).