Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity

Figure 5

Effect of ex vivo kidney preservation at 4°C, 19°C or 32°C on renal histological injuries. Representative pictures of kidney histology for the evaluation of tubular dilatation (dt), brush border loss (bbl), and cell detachment (cd) injuries by histological analysis (PAS coloration) in renal tissue from control time to 1 h, 6 h, and 24 h of organ preservation at 4°C, 19°C, and 32°C (n = 3 per group). Results are expressed as mean ± SEM of percentage of lesions by field; scores: 0 = no alteration, 0.5 = lesions <5%, 1 = lesions 6-10%, 2 = lesions between 11-25%, 3 = lesions between 26-50%, 4 = lesions between 51 and 75%, and 5 = lesions >76%. Statistical significance (, p<0.05) was calculated using nonparametric Kruskal-Wallis test + multiple comparison correction by Dunn’s posttest.