Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity

Figure 7

Effect of ex vivo kidney preservation at 4°C, 19°C, or 32°C on apoptosis. Left panel: representative pictures and histogram of the number of cleaved caspase 3 positive cells (mean of positive cells per field), as detected by immunohistofluorescence on kidney tissue after 6 h of preservation in UW at 4°C, 19°C and 32°C (n = 3). Right panel: representative pictures and histogram of number of cleaved caspase 3 positive cells (mean of positive cells per field), detected by immunohistofluorescence on kidney tissue after 24 h of preservation in UW at 4°C, 19°C, and 32°C (measure on 10 fields per slide. n = 3 per group).