Analysis of Expression and Single Nucleotide Polymorphisms of INHA Gene Associated with Reproductive Traits in Chickens

Table 1

Primer pairs used to screen the INHA gene polymorphism.

Name	Sequences (5＇-3＇)	Binding regions	Product size (bp)	Tm (°C)
P1	F-TATTCACGGCGAGCAGACA	Exon 1	562	58.7
	R-CCTCAGCCCTCCCCATCT
P2	F-ATCCCACAGCCCCAAGACC	Exon 2	783	61.0
	R-GGCAGTAGTGGAAAACGAAGC
P3	F-CACTGGACCGTGTTTGACTTCG	Exon 2 Partial	589	51.5
	R-GGGATGGGCTCATCATCTGG
P4	F-CGGGAACTGTGCCGAAGG	5'UTR	749	58.7
	R-GCACCCCGCTGCATAACC
P5	F-TGTCCCAAACTCTGTCCAATG	3'UTR	707	56.5
	R-CTCAAATGCTCCAGCACCC

UTR: untranslated region.