Curcumin Inhibits Proliferation of Epstein–Barr Virus-Associated Human Nasopharyngeal Carcinoma Cells by Inhibiting EBV Nuclear Antigen 1 Expression

Figure 4

Effects of curcumin on transcription, expression, and half-time of EBNA1 in HONE1 and HK1-EBV cells. (a) HONE1 and HK1-EBV cells treated with DMSO (vehicle control) or curcumin (5 μM and 10 μM), for 48 h. (b) Statistical analysis of the expression levels of EBNA1 in the HONE1 and HK1-EBV cells. (c) HeLa cells transfected with pSG5 or pSG5-EBNA1, followed by the treatment of curcumin (5 μM and 10 μM) for 44 h (d) HONE1 and HK1-EBV cells treated with DMSO (vehicle control) or curcumin (5 μM and 10 μM), for 24 h (e) HeLa cells cotransfected with pRL-TK and pGL3.0-Qp, followed by the treatment with DMSO (vehicle control) or curcumin (5 μM and 10 μM). The activity of the promoter was determined by the Dual-Luciferase reporter assay. The HONE1 (f) and HK1-EBV (g) cells treated with DMSO (vehicle control) or curcumin (10 μM), in the presence or absence of CHX (50 μg/ml). The HONE1 (h) and HK1-EBV (i) cells treated with DMSO (vehicle control) or curcumin (10 μM), in the presence or absence of MG-132 (50 μg/ml). Transcription levels of EBNA1 were determined by quantitative real-time PCR. Western blot analysis was performed to detect the protein expression levels. Compared with the vehicle control (0.006% DMSO) group, , .

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